Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Mus musculus | |
CO | partially purified rat cerebellum enzyme | Rattus norvegicus | |
CO/O2 | 80%:20%, mixture | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | mouse macrophage enzyme: cytochrome P-450 type hemoprotein | Mus musculus | |
Iron | protoporphyrin IX heme | Mus musculus | |
Iron | protoporphyrin IX heme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Mus musculus | soluble cytochrome P-450 enzyme in eukaryotes | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | soluble cytochrome P-450 enzyme in eukaryotes | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP-agarose affinity chromatography | Mus musculus |
2',5'-ADP-agarose affinity chromatography | Rattus norvegicus |
partial | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | possible mechanism | Mus musculus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | possible mechanism | Rattus norvegicus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | cytochrome P-450 enzyme | Mus musculus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | cytochrome P-450 enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
macrophage | - |
Mus musculus | - |
macrophage | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | specific for NADPH, 5-electron oxidation of L-arginine | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | specific for NADPH, 5-electron oxidation of L-arginine | Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | soluble cytochrome P-450 enzyme in eukaryotes | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | soluble cytochrome P-450 enzyme in eukaryotes | Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | significant amino acid sequence homology to NADPH-cytochrome P-450-type hemoprotein | Mus musculus |
More | significant amino acid sequence homology to NADPH-cytochrome P-450-type hemoprotein | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Mus musculus | |
5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus |